cloning相关论文
Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and
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Difference in DNA sequences in SSU rDNA variable regions among pathogens isolated from different epi
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目的分析C666-1放射存活亚克隆的放射敏感性并分析其GRP78蛋白的表达水平。方法首先使用大剂量8Gy的X射线照射C666-1，三个存活亚克......
Cloning and Homology Comparison of S Gene for Isolate TH-98 of Porcine Transmissible Gastroenteritis
TH-98 isolate of transmissible gastroenteritis virus (TGEV) was propagated and harvested onswine testicle (ST) monolayer......
目的克隆日本血吸虫原肌球蛋白编码基因,并在大肠杆菌中表达.方法抽提日本血吸虫(大陆株)成虫总RNA,经逆转录-聚合酶链反应(RT-PCR......
Cloning and Expressing of a Gene Encoding Cytosolic Copper/Zinc Superoxide Dismutase in the Upland C
In this study, a gene encoding a superoxide dismutase (SOD) was cloned from senescent leaves of cotton (Gossypium hirsut......
目的 克隆墨西哥利什曼原虫(L.mer)WR972株的无鞭毛体蛋白(amastin)的编码基因，并对其同源核苷酸序列进行分析。方法 根据已克隆......
BACKGROUND: Research of transgene brings hope for gene therapy of various diseases; in addition, some projects have been......
Construction of eukaryotic expression vector with brain-derived neurotrophic factor receptor trkB ge
Objective: To construct an eukaryotic expression vector carrying rat brain-derived neurotrophic factor receptor trkB gen......
题目 20世纪50年代以来，动物克隆不断取得进展。1996年，克隆羊“多莉”出世，然后野牛、猫、狗等也先后被克隆。动物克隆是科学进步的......
AIM:To clone and express the human colon mast cellcarboxypeptidase(MC-CP)gene.METHODS:Total RNA was extracted from colo......
Background and Objective: Since the proposal of the tumor stem cell hypothesis, considerable interest has been devoted t......
Objective To construct eukaryotic expression vector of HPV18 L1-E6, E7 chimeric gene and examine the humoral and cellula......
Objective: To facilitate manipulation of gene expression in different host cells, we used pEGFP-N1 as backbone to constr......
The Human Genome Project was launched at the end of the 1980s.Since then,the cloning and identification of functional ge......
一、多项选择 1. It is amazing that many sea animals such as dolphins can_________ high speeds in water which man cann......
自从20世纪50年代以来，动物克隆不断取得进展，青蛙、老鼠先后被成功克隆。1996年，克隆羊“多莉”出世，野牛、猫、狗等也先后被克隆。动......
摘 要 本文由当今中学英语教学现状（词汇教学的困境、难以提高的综合运用能力、母语负迁移的影响）引发感想，认为语块可以有效提高......
【摘要】在新课改的规定中，对高中英语课程的总目标做出了更明确的指导，不仅要求提高学生的语言交际能力，还进一步强调了综合语言应用......
本文围绕着“克隆人:不可逾越的伦理禁区”这一主题,首先从技术层面阐述了克隆人实验所要面临的无可逾越的伦理难关;然后具体分析......
MDM2(murine double minute)是肿瘤抑制因子P53最重要的负性调控因子。为了解家蚕是否存在mdm2基因,利用人和小鼠的mdm2基因作为电......
The principles and characteristics of several novel molecular markers, SCAR (Sequence Characterized Amplified Region), I......
目的 克隆人角蛋白19(CK19)基因,原核表达重组蛋白并纯化,为建立一种新的非小细胞肺癌诊断方法奠定基础.方法 从宫颈癌HeLa细胞株......
The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stress......
A homologue of flowering locus T gene,designated StFT,was isolated from Solanum tuberosum by reverse transcriptasepolyme......
Selecting beneficial DNA variants is the main goal of animal breeding.However,this process is inherently inefficient bec......
Cloning and expression analysis of Nuclear receptor genes (FTZ-F1,TRα and TRβ)in black rockfish (seb
The Sebastes schlegelii is distributed along the coast of Japan, Korea and China. It is well established that rock fish ......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Cloning and characterization of gene-resistant analogs (RGAs) involved in rust (Puccinia psidii) res
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Cloning and Homology Comparison of S Gene for Isolate TH-98 of Porcine Transmissible Gastroenteritis
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Cloning of a novel phytase from an anaerobic rumen bacterium, Mitsuokella jalaludinii, and its expre
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
很多研究者试图建立体内外模型,模拟HCV复制,都难以达到目的.直到2005年在1例暴发性肝炎患者体内分离到HCV 2a株,具有很强的复制能......
目的测定恶性疟原虫FCC1/HN株exp-1基因序列.方法根据exp-1基因已知序列设计合成1对引物,用PCB技术从FCC1/HN株基因组DNA中扩增exp......
根据平榛花芽转录本高通量测序的结果，采用RACE-PCR法克隆到1个平榛WRKY基因，全长1273bp，推断其编码317个氨基酸，命名为ChWRKYl。序列......
目的 克隆穿孔素基因启动子区域(PRF1),并对PRF1进行体外区域性高甲基化,为探讨穿孔素基因调控序列高甲基化能否引起穿孔素表达下......
Cloning and RNAi Construction of a LEAFY Homologous Gene from Populus tomentosa and Preliminary Stud
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase c
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
目的 克隆抗日本脑炎病毒单克隆抗体2F2重链可变区(VH)基因.方法 从分泌抗日本脑炎病毒单克隆抗体2F2的杂交瘤细胞中提取总RNA,通......
Cloning of an APETALA3homologous gene (PtAP3) from Populus tomentosa and genetic transformation of i
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
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拟南芥氨基酸透性酶1（ AAP1，也叫NAT1）是植物中第一个被分离的氨基酸转运蛋白，在拟南芥主根、侧根、根毛及根表皮细胞中均有增加氨基酸......
以五年生人参根组织为试验材料,提取总RNA,反转录合成cDNA,利用RT-PCR法对人参鲨烯环氧酶(SQE)基因的cDNA进行克隆及序列分析,初步......
结核分枝杆菌保护性抗原TB10?4属于结核分枝杆菌早期分泌蛋白中的ESAT?6家族，其免疫原性等与ESAT?6相当或者更佳，具有十分重要的研究......
体细胞克隆牛生产效率受实验室环节和胚胎移植环节等多种因素的影响。依据前期试验结果，重点分析了胚胎移植数量、季节变化和胚胎移......
